| Project Title: | Development of Gene Transfer Protocols for Soybeans |
| Project Leader: | Nancy A. Reichert, Plant and Soil Sciences, MAFES,
Mississippi State University |
| Other Participant: | Jeff M. Tyler, Soybean Prod. Res. Unit, USDA, ARS, Stoneville, MS |
Use developed transformation, selection and regeneration protocols to produce soybean tissues and plants for screening.
Numerous tissues were initiated and maintained for eventual screening after prolonged periods of selection. Hundreds of tissues are still being maintained and are at various stages of development. The soybean genotypes we have concentrated on include mat
urity groups IV (Crawford), V (Hill, York, DT9515091, DT9515550) and VI (P1398469). The DT lines are promising breeding lines from Dr. Jeff Tyler's breeding program.
Objective 2
Screen soybean tissues and plants for presence of introduced foreign DNA for confirmation of transformation.
DNA was extracted from approximately 20 different putatively transgenic soybean shoots and analyzed via PCR, but none contained foreign DNA (not transformed). Other tissues were recently screened via histochemical assays. Shoot tips or leaves from 5 smal
l shoots (line P1398469) expressed the P-glucuronidase (GUS) gene product, indicating the presence of foreign DNA (Figure 2). (Those tissues had been bombarded in June, 1997 and last week, the regenerated shoots were finally large enough to sacrifice a po
rtion of each for analysis.)
Objective 3
Grow transgenic soybean plants in the greenhouse for generation of seeds for future field and molecular analyses.
Unfortunately, our project did not get this far to accomplish this goal last year.
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